CD31 Monoclonal antibody

CD31 Monoclonal Antibody for WB, IF, IHC, ELISA
Cat No. 66065-1-Ig

产品说明书

CloneNo. 2A1E2

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IF, IHC, ELISA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, THP-1 cells, human placenta tissue
Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66065-1-Ig targets CD31 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human
免疫原 CD31 fusion protein Ag1787 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 platelet/endothelial cell adhesion molecule
别名
计算分子量 83 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC022512
基因名称 CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
RRIDAB_11042590
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16284
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
WB protocol for CD31 antibody 66065-1-IgDownload protocol
IHC protocol for CD31 antibody 66065-1-IgDownload protocol
IF protocol for CD31 antibody 66065-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nature

Construction of a human cell landscape at single-cell level.

Authors - Xiaoping Han

Biomaterials

An iRGD-conjugated prodrug micelle with blood-brain-barrier penetrability for anti-glioma therapy.

Authors - Lu Lu
humanIF

Small

Airflow-Assisted 3D Bioprinting of Human Heterogeneous Microspheroidal Organoids with Microfluidic Nozzle.

Authors - Haiming Zhao
humanIF

Theranostics

Single-cell landscape reveals active cell subtypes and their interaction in the tumor microenvironment of gastric cancer.

Authors - Yumin Li
IF

Theranostics

Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis.

Authors - Ke Feng
IHC

Cancer Lett

CD44(+) tumor cells promote early angiogenesis in head and neck squamous cell carcinoma.

Authors - Nils Ludwig
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