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验证数据展示

  • IHC staining of human kidney using 12518-1-AP

    IHC staining of human kidney using 12518-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12518-1-AP (MAP17 / PDZK1IP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 12518-1-AP

    IHC staining of human kidney using 12518-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12518-1-AP (MAP17 / PDZK1IP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 12518-1-AP

    IF Staining of PC-3 using 12518-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using MAP17 / PDZK1IP1 antibody (12518-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MAP17 / PDZK1IP1 Polyclonal antibody

MAP17 / PDZK1IP1 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 12518-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

Protein DD96, PDZK1IP1, PDZK1-interacting protein 1, MAP17, 17 kDa membrane-associated protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12518-1-AP targets MAP17 / PDZK1IP1 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MAP17 / PDZK1IP1 fusion protein Ag3206 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PDZK1 interacting protein 1
别名 Protein DD96, PDZK1IP1, PDZK1-interacting protein 1, MAP17, 17 kDa membrane-associated protein
计算分子量 114 aa, 12 kDa
GenBank蛋白编号BC012303
基因名称 MAP17
Gene ID (NCBI) 10158
RRIDAB_3085395
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13113
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDZK1-interacting protein 1(PDZK1IP1), also known as MAP17, is a membrane-associated protein. MAP17 / PDZK1IP1 is overexpressed in a variety of human carcinomas, and overexpression of MAP17 / PDZK1IP1 protein is strongly correlated with the progression of prostate and ovarian carcinomas (PMID: 17426052). MAP17 / PDZK1IP1 has been proven to be an oncogene for tumorigenesis and development of papillary thyroid cancer (PTC) (PMID: 36057192; PMID: 35727731).

实验方案

Product Specific Protocols
IHC protocol for MAP17 / PDZK1IP1 antibody 12518-1-APDownload protocol
IF protocol for MAP17 / PDZK1IP1 antibody 12518-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols