验证数据展示
经过测试的应用
| Positive WB detected in | mouse pancreas tissue, rat pancreas tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31458-1-AP targets PDIA2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | PDIA2 fusion protein Ag35301 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | protein disulfide isomerase family A, member 2 |
| 别名 | PDA2, PDI, PDIP, PDIR, Protein disulfide isomerase A2 |
| 计算分子量 | 58 kDa |
| 观测分子量 | 60-65 kDa |
| GenBank蛋白编号 | NM_006849.2 |
| 基因名称 | PDIA2 |
| Gene ID (NCBI) | 64714 |
| RRID | AB_3669994 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q13087 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDIA2 (Protein disulfide-isomerase A2), a member of PDI family, is an ER protein chaperone that catalyzes the formation and breakage of disulfide bonds between cysteine residues and can inhibit aggregation of misfolded proteins by catalyzing rearrangement of -S-S- bonds in proteins. (PMID: 23486466)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDIA2 antibody 31458-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |