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验证数据展示

  • IHC staining of mouse testis using 24564-1-AP

    IHC staining of mouse testis using 24564-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24564-1-AP (PCOTH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 24564-1-AP

    IHC staining of mouse testis using 24564-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24564-1-AP (PCOTH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 24564-1-AP

    IF Staining of PC-3 using 24564-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using PCOTH antibody (24564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

PCOTH Polyclonal antibody

PCOTH Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 24564-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

PCOTH, prostate collagen triple helix, Protein PCOTH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inmouse testis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24564-1-AP targets PCOTH in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20092

Product name: Recombinant human PCOTH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 8-107 aa of BC142999

Sequence: GTSEEGNLLSTVSPTVKALFGKTRVSPIFPFSPRSPFQPLIPRTPGSPWGPVGPASPLGPGFPIGPMGPGKPVGPKGPMLPLGPSGPVGPTSPLFPFCP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 prostate collagen triple helix
别名 PCOTH, prostate collagen triple helix, Protein PCOTH
计算分子量 107 aa, 11 kDa
GenBank蛋白编号BC142999
基因名称 PCOTH
Gene ID (NCBI) 542767
RRIDAB_2918084
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ58A44
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCOTH may be involved in growth and survival of prostate cancer cells through the TAF-Ibeta pathway.

实验方案

Product Specific Protocols
IHC protocol for PCOTH antibody 24564-1-APDownload protocol
IF protocol for PCOTH antibody 24564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols