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PCOTH Polyclonal antibody

PCOTH Polyclonal Antibody for IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 24564-1-AP

Print datasheet

Synonyms

PCOTH, prostate collagen triple helix, Protein PCOTH

验证数据展示

  • IHC staining of mouse testis using 24564-1-AP

    IHC staining of mouse testis using 24564-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24564-1-AP (PCOTH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 24564-1-AP

    IHC staining of mouse testis using 24564-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24564-1-AP (PCOTH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of PC-3 using 24564-1-AP

    IF Staining of PC-3 using 24564-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using PCOTH antibody (24564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse testis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24564-1-AP targets PCOTH in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, ELISA
Tested Reactivity human, mouse
Immunogen PCOTH fusion protein Ag20092 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name prostate collagen triple helix
Synonyms PCOTH, prostate collagen triple helix, Protein PCOTH
Calculated Molecular Weight 107 aa, 11 kDa
GenBank Accession NumberBC142999
Gene Symbol PCOTH
Gene ID (NCBI) 542767
RRIDAB_2918084
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ58A44
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCOTH may be involved in growth and survival of prostate cancer cells through the TAF-Ibeta pathway.

实验方案

Product Specific Protocols
IHC protocol for PCOTH antibody 24564-1-APDownload protocol
IF protocol for PCOTH antibody 24564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols