ChromoTek PARP1-Chromobody® plasmid (TagRFP)

DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagRFP.




IF, Live cell imaging

Vector type

Mammalian expression vector



Cat No : xcr


Chromobody, PARP1 Chromobody

Product Information

DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagRFP.

DescriptionThe PARP1-Chromobody visualizes endogenous PARP1 in real time in live cells.

• Easily transfect your cells

• Use PARP1-Chromobody® as marker of DNA damage after microirradiation

• PARP1-Chromobody® does not affect cell viability

ApplicationsLive cell imaging
SpecificityPoly(ADP-ribose) polymerase 1 (PARP1), tested in human cells.
Does not bind to PARP2, PARP3, andPARP 9
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
Codon usageMammalian
SequenceWith the PARP1-Chromobody plasmid you receive the sequence information of the alpaca antibody to PARP1 fused to TagGFP2 or TagRFP, as well as the full vector sequence.
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy
Storage ConditionShipped at ambient temperature. Store at -20 °C



Nat Commun

SPINDOC binds PARP1 to facilitate PARylation.

Authors - Fen Yang

Proc Natl Acad Sci U S A

Rapid recruitment of p53 to DNA damage sites directs DNA repair choice and integrity.

Authors - Yu-Hsiu Wang

J Cell Sci

Local inhibition of rRNA transcription without nucleolar segregation after targeted ion irradiation of the nucleolus.

Authors - Christian Siebenwirth

J Biophotonics

Chromatin nanoscale compaction in live cells visualized by acceptor-to-donor ratio corrected Förster resonance energy transfer between DNA dyes.

Authors - Simone Pelicci