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- KD/KO Validated
PARK2/Parkin Polyclonal antibody
PARK2/Parkin Polyclonal Antibody for IF/ICC,IF-P, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (6)
Applications
IF/ICC,IF-P, IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
206
验证数据展示
经过测试的应用
Positive WB detected in | C6 cells, HEK-293 cells, mouse brain tissue, SH-SY5Y cells, PC-12 cells, mouse liver tissue |
Positive IHC detected in | mouse kidney tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | SH-SY5Y cells, mouse brain tissue, mouse heart tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14060-1-AP targets PARK2/Parkin in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC,IF-P, IHC, WB, ELISA |
Cited Applications | WB, IP, IF, IHC, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, cattle, ducks, rabbit, pig, bovine |
Immunogen | PARK2/Parkin fusion protein Ag5092 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | Parkinson disease (autosomal recessive, juvenile) 2, parkin |
Synonyms | AR JP, LPRS2, PARK2, PARK2/Parkin, Parkin, Parkinson disease protein 2, PDJ, PRKN |
Calculated Molecular Weight | 52 kDa |
Observed Molecular Weight | 42-52 kDa |
GenBank Accession Number | BC022014 |
Gene Symbol | PARK2 |
Gene ID (NCBI) | 5071 |
RRID | AB_2878005 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O60260 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PARK2/Parkin antibody 14060-1-AP | Download protocol |
IHC protocol for PARK2/Parkin antibody 14060-1-AP | Download protocol |
IF protocol for PARK2/Parkin antibody 14060-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Augmented temperature fluctuation aggravates muscular atrophy through the gut microbiota | ||
Acta Pharm Sin B Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy. | ||
Acta Pharm Sin B Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy | ||
Autophagy Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways. | ||
Redox Biol Genistein mitigates senescence of bone marrow mesenchymal stem cells via ERRα-mediated mitochondrial biogenesis and mitophagy in ovariectomized rats | ||