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PAK7 Polyclonal antibody

PAK7 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse , rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 27847-1-AP

Print datasheet

Synonyms

KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK5, PAK7

验证数据展示

  • WB analysis using 27847-1-AP

    WB analysis using 27847-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27847-1-AP (PAK7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 27847-1-AP

    IP experiment of mouse brain using 27847-1-AP

    IP result of anti-PAK7 (IP:27847-1-AP, 4ug; Detection:27847-1-AP 1:3000) with mouse brain tissue lysate 1280 ug.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27847-1-AP targets PAK7 in WB, IP, ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Applications IP, WB, ELISA
Tested Reactivity Human, mouse , rat
Immunogen PAK7 fusion protein Ag27283 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name p21 protein (Cdc42/Rac)-activated kinase 7
Synonyms KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK5, PAK7
Calculated Molecular Weight 719 aa, 81 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC024179
Gene Symbol PAK7
Gene ID (NCBI) 57144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P286
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

p21-activated kinase 7 (PAK7), also known as PAK5, is a member of the p21-activated kinase family (Paks). PAK7 is mainly expressed in the brain and testis and has a molecular mass of 81 kDa. It plays an important role in tumorigenesis, invasion and metastasis (PMID: 19165420).

实验方案

Product Specific Protocols
WB protocol for PAK7 antibody 27847-1-APDownload protocol
IP protocol for PAK7 antibody 27847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols