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验证数据展示

  • WB analysis using 27847-1-AP

    WB analysis using 27847-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27847-1-AP (PAK7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 27847-1-AP

    IP experiment of mouse brain using 27847-1-AP

    IP result of anti-PAK7 (IP:27847-1-AP, 4ug; Detection:27847-1-AP 1:3000) with mouse brain tissue lysate 1280 ug.

PAK7 Polyclonal antibody

PAK7 Polyclonal Antibody for WB, IP, ELISA
Cat No. 27847-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse , rat

应用

WB, IP, ELISA

别名

KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK5, PAK7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27847-1-AP targets PAK7 in WB, IP, ELISA applications and shows reactivity with Human, mouse , rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 Human, mouse , rat
免疫原 PAK7 fusion protein Ag27283 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 p21 protein (Cdc42/Rac)-activated kinase 7
别名 KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK5, PAK7
计算分子量 719 aa, 81 kDa
观测分子量80 kDa
GenBank蛋白编号BC024179
基因名称 PAK7
Gene ID (NCBI) 57144
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P286
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

p21-activated kinase 7 (PAK7), also known as PAK5, is a member of the p21-activated kinase family (Paks). PAK7 is mainly expressed in the brain and testis and has a molecular mass of 81 kDa. It plays an important role in tumorigenesis, invasion and metastasis (PMID: 19165420).

实验方案

Product Specific Protocols
WB protocol for PAK7 antibody 27847-1-APDownload protocol
IP protocol for PAK7 antibody 27847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols