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PAI-1 Polyclonal antibody

PAI-1 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 25522-1-AP

Print datasheet

Synonyms

PAI, PAI 1, PAI1, PLANH1, Serpin E1, SERPINE1

验证数据展示

  • WB analysis of RAW 264.7 using 25522-1-AP

    WB analysis of RAW 264.7 using 25522-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 25522-1-AP (PAI-1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRAW 264.7 cells, human plasma tissue
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SERPINE1 Ready-To-Use IHC Kit. SERPINE1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

The immunogen of 25522-1-AP is PAI-1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen PAI-1 fusion protein Ag22411 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Synonyms PAI, PAI 1, PAI1, PLANH1, Serpin E1, SERPINE1
Calculated Molecular Weight 402 aa, 45 kDa
GenBank Accession NumberBC010860
Gene Symbol SERPINE1
Gene ID (NCBI) 5054
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05121
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.

实验方案

Product Specific Protocols
WB protocol for PAI-1 antibody 25522-1-APDownload protocol
IF protocol for PAI-1 antibody 25522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Cell Dev Biol

The predictive effect of immune therapy and chemotherapy under T cell-related gene prognostic index for Gastric cancer

Authors - Jingyao Chen
View Article