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验证数据展示

  • WB analysis using 25582-1-AP

    WB analysis using 25582-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25582-1-AP (PABPC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 25582-1-AP

    IF Staining of HeLa using 25582-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PABPC5 antibody (25582-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

PABPC5 Polyclonal antibody

PABPC5 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25582-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Polyadenylate-binding protein 5, Poly(A)-binding protein 5, Poly(A) binding protein 5, PABP-5, PABP5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, SKOV-3 cells, U-251 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25582-1-AP targets PABPC5 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 PABPC5 fusion protein Ag22216 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly(A) binding protein, cytoplasmic 5
别名 Polyadenylate-binding protein 5, Poly(A)-binding protein 5, Poly(A) binding protein 5, PABP-5, PABP5
计算分子量 382 aa, 43 kDa
观测分子量37-43 kDa, 70 kDa
GenBank蛋白编号BC063113
基因名称 PABPC5
Gene ID (NCBI) 140886
RRIDAB_3085806
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96DU9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PABPC5 antibody 25582-1-APDownload protocol
IF protocol for PABPC5 antibody 25582-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols