验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, NIH/3T3 cells, human heart tissue, rat skin tissue, HT-1080 cells, BGC-823 cells, PC-3 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human pancreas cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12658-1-AP targets P4HA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3292 Product name: Recombinant human P4HA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 236-534 aa of BC034998 Sequence: PEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | prolyl 4-hydroxylase, alpha polypeptide I |
| 别名 | EC:1.14.11.2, P4HA, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, Prolyl 4-hydroxylase subunit alpha-1 |
| 计算分子量 | 534 aa, 61 kDa |
| 观测分子量 | 61 kDa |
| GenBank蛋白编号 | BC034998 |
| 基因名称 | P4HA1 |
| Gene ID (NCBI) | 5033 |
| RRID | AB_2283162 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P13674 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
P4HA1 (Prolyl 4-hydroxylase subunit alpha-1) is also known as P4HA and belongs to the P4HA family, which plays a central role in collagen synthesis. P4HA1 catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. The gene encodes a polypeptide of 517 amino acid residues and a signal peptide of 17 amino acids and the full length protein has two glycosylation sites (uniprot). It can exist as a heteromer, dimer or tetramer (refer to: GENATLAS). P4HA1 has some isoforms with the MW 61-63 kDa and 57-59 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for P4HA1 antibody 12658-1-AP | Download protocol |
| IHC protocol for P4HA1 antibody 12658-1-AP | Download protocol |
| IP protocol for P4HA1 antibody 12658-1-AP | Download protocol |
| WB protocol for P4HA1 antibody 12658-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Differ Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation | ||
J Exp Med IL-17-induced HIF1α drives resistance to anti-PD-L1 via fibroblast-mediated immune exclusion. | ||
Oncotarget The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression.
| ||
Mol Oncol Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker of poor prognosis in primary melanomas, and its depletion inhibits melanoma cell invasion and disrupts tumor blood vessel walls.
| ||
Aging (Albany NY) Identification of a novel glycolysis-related gene signature for predicting the prognosis of osteosarcoma patients.
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