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验证数据展示

  • WB analysis using 13497-1-AP (same clone as 13497-1-PBS)

    WB analysis using 13497-1-AP (same clone as 13497-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13497-1-AP (OTX2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13497-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 13497-1-AP (same clone as 13497-1-PBS)

    IHC staining of human gliomas using 13497-1-AP (same clone as 13497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 13497-1-AP (OTX2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13497-1-PBS in a different storage buffer formulation.

  • IP experiment of Y79 using 13497-1-AP (same clone as 13497-1-PBS)

    IP experiment of Y79 using 13497-1-AP (same clone as 13497-1-PBS)

    IP result of anti-OTX2 (IP:13497-1-AP, 3ug; Detection:13497-1-AP 1:1500) with Y79 cells lysate 2400ug. This data was developed using the same antibody clone with 13497-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 13497-1-AP (same clone as 13497-1-PBS)

    FC experiment of HepG2 using 13497-1-AP (same clone as 13497-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human OTX2 (13497-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 13497-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

OTX2 Polyclonal antibody, PBS Only

OTX2 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 13497-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), IP, Indirect ELISA

别名

MCOPS5, Homeobox protein OTX2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

13497-1-PBS targets OTX2 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 OTX2 fusion protein Ag4323 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 orthodenticle homeobox 2
别名 MCOPS5, Homeobox protein OTX2
计算分子量 297 aa, 32 kDa
观测分子量 31-35 kDa
GenBank蛋白编号BC032579
基因名称 OTX2
Gene ID (NCBI) 5015
RRIDAB_2157176
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP32243
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The orthodenticle homeobox 2, encoded by OTX2 gene, is a key transcription factor in developmental processes. In particular, it is required for the early specification of the brain and the embryonic development of sensory organs, including the, pineal gland, pituitary gland, inner part of the ear, eyes, and optic nerve. In later stages, it is important for maintaining intact retina and brain function. In addition, it acts as a transcriptional repressor and a gatekeeper of myogenic and neuronal differentiation in medulloblastoma cells. OTX2 binds to the MyoD1 core enhancer through its homeobox domain and the remarkable repressor activity exhibited by the homeobox domain renders OTX2 transcriptionally repressive