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- KD/KO Validated
OPTN Polyclonal antibody
OPTN Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
FC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse testis tissue, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
产品信息
10837-1-AP targets OPTN in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IHC, IP, WB, ELISA |
| Cited Applications | IF, IHC, IP, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Immunogen | OPTN fusion protein Ag1272 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | optineurin |
| Synonyms | E3 14.7K interacting protein, FIP 2, FIP2, GLC1E, HIP 7, HIP7, Huntingtin yeast partner L, HYPL, NEMO related protein, NRP, optineurin, OPTN, TFIIIA INTP |
| Calculated molecular weight | 66 kDa |
| Observed molecular weight | 66 kDa, 70-74 kDa |
| GenBank accession number | BC013876 |
| Gene symbol | OPTN |
| Uniprot | Q96CV9 |
| Gene ID (NCBI) | 10133 |
| RRID | AB_2156665 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E). Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. OPTN has 3 isoforms with MW 66,65 and 60 kDa (refer to UniProt). Catalog#10837-1-AP recognises 66 and 70-74 kDa band, and the additional 70-74 kDa band due to phosphorylation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for OPTN antibody 10837-1-AP | Download protocol |
| IHC protocol for OPTN antibody 10837-1-AP | Download protocol |
| IP protocol for OPTN antibody 10837-1-AP | Download protocol |
| FC protocol for OPTN antibody 10837-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling. | ||
Mol Cell Loss of TAX1BP1-Directed Autophagy Results in Protein Aggregate Accumulation in the Brain.
| ||
Nat Commun TDP-43 and PINK1 mediate CHCHD10S59L mutation-induced defects in Drosophila and in vitro. | ||
Nat Commun Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration. |
