• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

OGFOD1 Polyclonal antibody

OGFOD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 30800-1-AP

Print datasheet

Synonyms

FLJ10826, KIAA1612, OGFOD1, TPA1

验证数据展示

  • WB analysis using 30800-1-AP

    WB analysis using 30800-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30800-1-AP (OGFOD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, DU 145 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30800-1-AP targets OGFOD1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen OGFOD1 fusion protein Ag34034 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 2-oxoglutarate and iron-dependent oxygenase domain containing 1
Synonyms FLJ10826, KIAA1612, OGFOD1, TPA1
Calculated Molecular Weight 542 aa, 63 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC032919
Gene Symbol OGFOD1
Gene ID (NCBI) 55239
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N543
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1 (OGFOD1) is a proly hydroxylase which belongs to 2-OG-Fe (II) dioxygenase family, it also namely KIAA1612 and TPA1. OGFOD1 is expressed in many tissues, mainly located in the nucleoplasm. It increased in many cancers and regulates both transcription and translation. The apparent molecular weight is larger than the calculated molecular weight of 63 kDa, which is likely due to posttranslational modification, presumably by glycosylation. (PMID:34298635 and 20579638)

实验方案

Product Specific Protocols
WB protocol for OGFOD1 antibody 30800-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols