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验证数据展示

  • IHC staining of human gliomas using 10975-2-AP (same clone as 10975-2-PBS)

    IHC staining of human gliomas using 10975-2-AP (same clone as 10975-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NR4A2 antibody included
  • WB analysis using 10975-2-AP (same clone as 10975-2-PBS)

    WB analysis using 10975-2-AP (same clone as 10975-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

  • IF Staining of Customer Sample using 10975-2-AP (same clone as 10975-2-PBS)

    IF Staining of Customer Sample using 10975-2-AP (same clone as 10975-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded Customer Sample customer using Nurr1/NR4A2 antibody (10975-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10975-2-PBS in a different storage buffer formulation.

Nurr1/NR4A2 Polyclonal antibody, PBS Only

Nurr1/NR4A2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 10975-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

NURR1, NR4A2, Orphan nuclear receptor NURR1, NOT, Immediate-early response protein NOT

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NR4A2 Ready-To-Use IHC Kit. NR4A2 primary antibody included.

产品信息

10975-2-PBS targets Nurr1/NR4A2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 Nurr1/NR4A2 fusion protein Ag1418 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear receptor subfamily 4, group A, member 2
别名 NURR1, NR4A2, Orphan nuclear receptor NURR1, NOT, Immediate-early response protein NOT
计算分子量 66 kDa
观测分子量 66 kDa
GenBank蛋白编号BC009288
基因名称 Nurr1
Gene ID (NCBI) 4929
RRIDAB_2153760
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43354
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].