ChromoTek Nuclear Actin-Chromobody® plasmid (TagGFP)

DNA plasmid encoding for anti-Actin VHH (anti-actin Nanobody)with nuclear localization signal (NLS) fused to TagGFP2.

Specificity

Actin

Applications

IF, Live cell imaging

Vector type

Mammalian expression vector

Reporter

TagGFP2

Cat No : acg-n

Synonyms

Actin Chromobody, Chromobody



Product Information

DNA plasmid encoding for anti-Actin VHH (anti-actin Nanobody)with nuclear localization signal (NLS) fused to TagGFP2.

DescriptionThe nuclear Actin-Chromobody allows imaging of actin dynamics in real-time in living cells.

• Easily transfect your cells and non-invasively visualize actin cytoskeleton in real-time

• Target and trace microfilaments in your cell line of interest

• Use Actin-Chromobody as a marker of cellular morphology and as a probe for cellular motility

• Actin-Chromobody has no notable effect cell viability or migration

The nuclear Actin-Chromobody® plasmid has been published and described by Prof. Dr. Robert Grosse, Institute of Pharmacology, BPC, University of Marburg, Germany under license from ChromoTek:Matthias Plessner, Michael Melak, Pilar Chinchilla, Christian Baarlink, Robert Grosse (2015). Nuclear F-actin formation and reorganization upon cell spreading J Biol Chem. (Pubmed)
ApplicationsLive cell imaging
Visualizing actin dynamics
Actin as biomarker or control
SpecificityActin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii
ReporterTagGFP2
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
Codon usageMammalian
SelectionKan/Neo
Nuclear localization signal (NLS)Yes
SequenceWith the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to Actin fused to TagRFP or TagGFP2, as well as the full vector sequence.
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED
Storage ConditionShipped at ambient temperature. Store at -20 °C

Publications

ApplicationTitle

Nature

Cytoplasmic control of intranuclear polarity by human cytomegalovirus.

Authors - Dean J Procter

Nat Cell Biol

Nuclear F-actin counteracts nuclear deformation and promotes fork repair during replication stress.

Authors - Noa Lamm

Nat Commun

Wiskott-Aldrich syndrome protein forms nuclear condensates and regulates alternative splicing.

Authors - Baolei Yuan
IF

J Cell Sci

The prophase oocyte nucleus is a homeostatic G-actin buffer.

Authors - Kathleen Scheffler

J Cell Sci

Nuclear F-actin and lamin a antagonistically modulate nuclear shape.

Authors - Sampada Mishra

Genes Cells

Characterization of phalloidin-negative nuclear actin filaments in U2OS cells expressing cytoplasmic actin-EGFP.

Authors - Akira Nagasaki