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Nocodazole

CAS号

31430-18-9

分子式

C14H11N3O3S

主要靶点

Autophagy|Apoptosis|CRISPR/Cas9|Bcr-Abl|Microtubule Associated

仅限科研使用

Cat No : CM05137

Print datasheet

Synonyms

诺考达唑|R 17934|R17934|R-17934|Nocodazole|Oncodazole|Inhibitor|inhibit|microtubule|Microtubule Associated|Microtubule/Tubulin|MicrotubuleAssociated|BcrAbl|Bcr-Abl|Autophagy|Cas9|Apoptosis|Abl (E255K)|Abl|Abl (T315I)|CRISPR|CRISPR/Cas9

验证数据展示

  • CAS No.: 31430-18-9

    Nocodazole
    CAS#: 31430-18-9

产品信息

CAS号 31430-18-9
分子式 C14H11N3O3S
主要靶点 Autophagy|Apoptosis|CRISPR/Cas9|Bcr-Abl|Microtubule Associated
主要通路 DNA 损伤和修复|细胞骨架|血管生成|细胞骨架|蛋白酪氨酸激酶|凋亡|自噬
分子量 301.32
纯度 99.91%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
别名 诺考达唑|R 17934|R17934|R-17934|Nocodazole|Oncodazole|Inhibitor|inhibit|microtubule|Microtubule Associated|Microtubule/Tubulin|MicrotubuleAssociated|BcrAbl|Bcr-Abl|Autophagy|Cas9|Apoptosis|Abl (E255K)|Abl|Abl (T315I)|CRISPR|CRISPR/Cas9

靶点活性

Abl:0.21 μM|Abl (T315I):0.64 μM|Abl (E255K):0.53 μM

体内活性

方法：为检测体内抗肿瘤活性，将 Nocodazole (12 mg/kg，每周三次) 和 dexamethasone (2 mg/kg，每周两次) 腹腔注射给携带骨髓瘤 H929 的免疫缺陷小鼠，持续十五天。 结果：Nocodazole 与 dexamethasone 联合显著抑制骨髓瘤肿瘤生长并延长生存期。[3] 方法：为研究对肠粘膜炎的作用，将 Nocodazole (5 mg/kg) 和 ketoconazole (50 mg/kg) 腹腔注射给携带人结直肠癌肿瘤 COLO 205 的 nude 小鼠，每周三次，持续六周。 结果：ketoconazole 治疗六周后，Nocodazole 的抗肿瘤作用显著增强。[4]

体外活性

方法：红细胞用 Nocodazole (15-60 μg/mL) 处理 48 h，使用 Annexin-V-FITC 检测磷脂酰丝氨酸。 结果：Nocodazole 处理增加了暴露于红细胞的磷脂酰丝氨酸的百分比，在 30 μg/mL 时达到统计学显著性。[1] 方法：hESCs 细胞用 Nocodazole (100 ng/mL) 处理 16 h，使用 Flow Cytometry 分析细胞周期情况。 结果：Nocodazole 处理可以同步细胞周期 (G2/M 中 >90% 的细胞)，而细胞在释放后保持同步，并在细胞周期中均匀移动 24 h。在去除 Nocodazole 后 2 h，细胞进入 G1 期，其中 70% 的细胞在 4 h 后处于 G1 期，80%的 细胞在 12 h后处于 S 期。[2]

溶解度

DMSO:12.5 mg/mL (41.48 mM)

细胞实验

Nocodazole is dissolved in a final concentration of 0.05% DMSO. Proteins are loaded at 50 μg/lane and separated by 12% (w:v) sodium dodecyl sulfate-polyacrylamide gel electrophoresis, blotted, and probed with antibodies for cyclin E, p53, p21/CIP1, p27/KIP1, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), cyclin A, cyclin D1, cyclin D3, cyclin B, CDK2, CDK4, and cytochrome C. Immunoreactive bands are visualized by incubating with the colorigenic substrates nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate. The expression of GAPDH is used as the control for equal protein loading.

参考文献

1.Signoretto E, et al. Nocodazole Induced Suicidal Death of Human Erythrocytes. Cell Physiol Biochem. 2016;38(1):379-92.
2.Bai Y, Wang W, Li S, et al. C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex[J]. Molecular Cell. 2019.
3.Yiangou L, et al. Method to Synchronize Cell Cycle of Human Pluripotent Stem Cells without Affecting Their Fundamental Characteristics. Stem Cell Reports. 2019 Jan 8;12(1):165-179.
4.Feng R, et al. Targeting the microtubular network as a new antimyeloma strategy. Mol Cancer Ther. 2011 Oct;10(10):1886-96.
5.Wang YJ, et al. Ketoconazole potentiates the antitumor effects of nocodazole: In vivo therapy for human tumor xenografts in nude mice. Mol Carcinog. 2002 Aug;34(4):199-210.
6.Wang YJ, et al. Mol Carcinog. 2002, 34(4), 199-210.
7.Signoretto E, et al. Nocodazole Induced Suicidal Death of Human Erythrocytes. Cell Physiol Biochem. 2016;38(1):379-92.
8.Zhang JP, et al. Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9-mediated double-stranded DNA cleavage. Genome Biol. 2017 Feb 20;18(1):35.
9.Li X, Tang H, Huang X, et al. Rigidity‐Dependent Placental Cells Uptake of Silk‐Based Microcapsules[J]. Macromolecular bioscience. 2019: 1900105.
10.Reyes G X, Kolodziejczak A, Devakumar L J P S, et al. Ligation of newly replicated DNA controls the timing of DNA mismatch repair[J]. Current Biology.

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