验证数据展示
经过测试的应用
Positive WB detected in | U-87 MG cells |
Positive IP detected in | U-87 MG cells |
Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
27193-1-AP targets Neuropilin 2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
经测试反应性 | human |
免疫原 | Neuropilin 2 fusion protein Ag25622 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | neuropilin 2 |
别名 | NRP2, Neuropilin-2 |
观测分子量 | 120-130 kDa |
GenBank蛋白编号 | BC104770 |
基因名称 | Neuropilin 2 |
Gene ID (NCBI) | 8828 |
RRID | AB_3669588 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O60462 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Neuropilin 2 (NRP2) is a single-chain transmembrane glycoprotein that acts as a co-receptor of VEGF. NRP2 is mainly expressed in lymphatic and venous endothelial cells, and it is associated with the occurrence and development of several types of cancer. NRP2 is known to play a role in cardiovascular development, axon guidance, tumorigenesis, inflammation and cardiovascular disease.
实验方案
Product Specific Protocols | |
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WB protocol for Neuropilin 2 antibody 27193-1-AP | Download protocol |
IHC protocol for Neuropilin 2 antibody 27193-1-AP | Download protocol |
IP protocol for Neuropilin 2 antibody 27193-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |