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- KD/KO Validated
NUSAP1 Polyclonal antibody
NUSAP1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12024-1-AP targets NUSAP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | NUSAP1 fusion protein Ag2654 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | nucleolar and spindle associated protein 1 |
| Synonyms | ANKT, BM037, LNP, NuSAP, NUSAP1, PRO0310, PRO0310p1, Q0310, SAPL |
| Calculated Molecular Weight | 440 aa, 49 kDa |
| Observed Molecular Weight | 47-52 kDa |
| GenBank Accession Number | BC001308 |
| Gene Symbol | NUSAP1 |
| Gene ID (NCBI) | 51203 |
| RRID | AB_2157789 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXS6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NUSAP1, also named as ANKT, belongs to the NUSAP family. It is microtubule-associated protein with the capacity to bundle and stabilize microtubules. NUSAP1 may associate with chromosomes and promote the organization of mitotic spindle microtubules around them. NUSAP1 plays a role in connecting apoptosis with cell migration and it is a potentially involved in morphogenesis in vertebrates (PMID:21203972). NUSAP1 is an immunogenic antigen in 65% of patients with AML following allogeneic HCT and suggests a tumor antigen role(PMID:20053754 ). NuSAP1 protein level is tightly regulated by the APC/C ubiquitin ligase complex and NuSAP induces mitotic arrest dependent of its microtubule affinity (PMID:17618083). NUSAP1 is overexpressed in pituitary adenomas.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NUSAP1 antibody 12024-1-AP | Download protocol |
| IHC protocol for NUSAP1 antibody 12024-1-AP | Download protocol |
| IF protocol for NUSAP1 antibody 12024-1-AP | Download protocol |
| IP protocol for NUSAP1 antibody 12024-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gastroenterology microRNA 193a-5p Regulates Levels of Nucleolar- and Spindle-Associated Protein 1 to Suppress Hepatocarcinogenesis. | ||
Mol Cell Regulated degradation of spindle assembly factors by the anaphase-promoting complex. | ||
J Exp Med Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine. | ||
Acta Neuropathol Transcriptome analysis of MENX-associated rat pituitary adenomas identifies novel molecular mechanisms involved in the pathogenesis of human pituitary gonadotroph adenomas. |
