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验证数据展示

  • WB analysis using 24858-1-AP (same clone as 24858-1-PBS)

    WB analysis using 24858-1-AP (same clone as 24858-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24858-1-AP (NRBF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24858-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 24858-1-AP (same clone as 24858-1-PBS)

    IHC staining of human liver using 24858-1-AP (same clone as 24858-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24858-1-AP (NRBF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24858-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NRBF2 antibody included
  • IF Staining of HepG2 using 24858-1-AP (same clone as 24858-1-PBS)

    IF Staining of HepG2 using 24858-1-AP (same clone as 24858-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NRBF2 antibody (24858-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24858-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 24858-1-AP (same clone as 24858-1-PBS)

    IP experiment of HEK-293 using 24858-1-AP (same clone as 24858-1-PBS)

    IP result of anti-NRBF2 (IP:24858-1-AP, 4ug; Detection:24858-1-AP 1:2000) with HEK-293 cells lysate 2640ug. This data was developed using the same antibody clone with 24858-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NRBF2 Polyclonal antibody, PBS Only

NRBF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 24858-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Nuclear receptor-binding factor 2, NRBF-2, NRBF 2, COPR2, COPR1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NRBF2 Ready-To-Use IHC Kit. NRBF2 primary antibody included.

产品信息

24858-1-PBS targets NRBF2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 NRBF2 fusion protein Ag21403 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear receptor binding factor 2
别名 Nuclear receptor-binding factor 2, NRBF-2, NRBF 2, COPR2, COPR1
计算分子量 32 kDa
观测分子量 35-37 kDa
GenBank蛋白编号BC001345
基因名称 NRBF2
Gene ID (NCBI) 29982
RRIDAB_2879759
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96F24
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NRBF2 also termed as Nuclear receptor-binding factor 2or COPR is a 287 amino acid protein, which Interacts with PPARA, PPARD and PPARG. NRBF2 as transcription activator may modulate transcriptional activation by target nuclear receptors. NRBF2 is expressed in keratinocytes, liver and placenta. NRBF2, which exists as two isoforms due to alternative splicing, is localized to both the nucleus and the cytoplasm.