验证数据展示
经过测试的应用
Positive IHC detected in | human small intestine tissue, human placenta tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
19505-1-AP targets NPFFR2 in IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | neuropeptide FF receptor 2 |
别名 | G protein coupled receptor 74, GPR74, HLWAR77, neuropeptide FF receptor 2, NPFFR2 |
计算分子量 | 60 kDa |
GenBank蛋白编号 | NM_053036 |
基因名称 | NPFFR2 |
Gene ID (NCBI) | 10886 |
RRID | AB_2878584 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9Y5X5 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NPFFR2, also named as GPR74, NPFF2 and NPGPR, belongs to the G-protein coupled receptor 1 family. NPFFR2 is a receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides which also known as morphine-modulating peptides. NPFFr2 can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. NPFFR2 mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The antibody can recognize isoform1, isoform2 and isoform3 of NPFFR2.
实验方案
Product Specific Protocols | |
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IHC protocol for NPFFR2 antibody 19505-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |