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验证数据展示

  • IHC staining of human small intestine using 19505-1-AP

    IHC staining of human small intestine using 19505-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19505-1-AP (NPFFR2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 19505-1-AP

    IHC staining of human small intestine using 19505-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19505-1-AP (NPFFR2 Antibody) at dilution of 1:50 (under 40x lens).

NPFFR2 Polyclonal antibody

NPFFR2 Polyclonal Antibody for IHC, ELISA
Cat No. 19505-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

G protein coupled receptor 74, GPR74, G-protein coupled receptor 74, G-protein coupled receptor HLWAR77, HLWAR77

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman small intestine tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19505-1-AP targets NPFFR2 in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuropeptide FF receptor 2
别名 G protein coupled receptor 74, GPR74, G-protein coupled receptor 74, G-protein coupled receptor HLWAR77, HLWAR77
计算分子量 60 kDa
GenBank蛋白编号NM_053036
基因名称 NPFFR2
Gene ID (NCBI) 10886
RRIDAB_2878584
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5X5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NPFFR2, also named as GPR74, NPFF2 and NPGPR, belongs to the G-protein coupled receptor 1 family. NPFFR2 is a receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides which also known as morphine-modulating peptides. NPFFr2 can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. NPFFR2 mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The antibody can recognize isoform1, isoform2 and isoform3 of NPFFR2.

实验方案

Product Specific Protocols
IHC protocol for NPFFR2 antibody 19505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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