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验证数据展示

  • WB analysis using 31633-1-AP

    WB analysis using 31633-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31633-1-AP (NPEPPS antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 31633-1-AP

    IF Staining of A549 using 31633-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using NPEPPS antibody (31633-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

NPEPPS Polyclonal antibody

NPEPPS Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31633-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

AAP S, Cytosol alanyl aminopeptidase, MP100, NPEPPS, PSA

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31633-1-AP targets NPEPPS in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag36035

Product name: Recombinant human NPEPPS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 711-919 aa of BC065294

Sequence: GKLGKAGHKATLEEARRRFKDHVEGKQILSADLRSPVYLTVLKHGDGTTLDIMLKLHKQADMQEEKNRIERVLGATLLPDLIQKVLTFALSEEVRPQDTVSVIGGVAGGSKHGRKAAWKFIKDNWEELYNRYQGGFLISRLIKLSVEGFAVDKMAGEVKAFFESHPAPSAERTIQQCCENILLNAAWLKRDAESIHQYLLQRKASPPTV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aminopeptidase puromycin sensitive
别名 AAP S, Cytosol alanyl aminopeptidase, MP100, NPEPPS, PSA
观测分子量103 kDa
GenBank蛋白编号BC065294
基因名称 NPEPPS
Gene ID (NCBI) 9520
RRIDAB_3670057
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP55786
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NPEPPS, also known as PSA, is a member of the oxytocinase subfamily of the M1 aminopeptidase family. NPEPPS encodes puromycin-sensitive aminopeptidase, a zinc metallopeptidase that hydrolyzes amino acids from the N-terminus of its substrate. The protein has 2 isoforms and is located in the cytoplasm.

实验方案

Product Specific Protocols
IF protocol for NPEPPS antibody 31633-1-APDownload protocol
WB protocol for NPEPPS antibody 31633-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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