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验证数据展示

  • WB analysis using 27278-1-AP

    WB analysis using 27278-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27278-1-AP (NLGN4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 27278-1-AP

    IHC staining of mouse brain using 27278-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27278-1-AP (NLGN4X antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 27278-1-AP

    IHC staining of mouse brain using 27278-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27278-1-AP (NLGN4X antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NLGN4X Polyclonal antibody

NLGN4X Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27278-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

ASPGX2, AUTSX2, HLNX, HNLX, neuroligin 4, X linked

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman brain tissue, mouse brain tissue, rat brain tissue, pig brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27278-1-AP targets NLGN4X in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag2426

Product name: Recombinant human NLGN4X protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 469-816 aa of BC034018

Sequence: FYAFYHHCQSEMKPSWADSAHGDEVPYVFGIPMIGPTELFSCNFSKNDVMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVAWSKYNPKDQLYLHIGLKPRVRDHYRATKVAFWLELVPHLHNLNEIFQYVSTTTKVPPPDMTSFPYGTRRSPAKIWPTTKRPAITPANNPKHSKDPHKTGPEDTTVLIETKRDYSTELSVTIAVGASLLFLNILAFAALYYKKDKRRHETHRRPSPQRNTTNDIAHIQNEEIMSLQMKQLEHDHECESLQAHDTLRLTCPPDYTLTLRRSPDDIPLMTPNTITMIPNTLTGMQPLHTFNTFSGGQNSTNLPHGHSTTRV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuroligin 4, X-linked
别名 ASPGX2, AUTSX2, HLNX, HNLX, neuroligin 4, X linked
计算分子量 92 kDa
观测分子量90-100 kDa
GenBank蛋白编号BC034018
基因名称 NLGN4X
Gene ID (NCBI) 57502
RRIDAB_3085943
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N0W4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NLGN4X is a member of the type-B carboxylesterase/lipase protein family. The encoded protein belongs to a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. The encoded protein interacts with discs large homolog 4 (DLG4). Mutations in this gene have been associated with autism and Asperger syndrome. NLGN4X has two isoforms with predicted MW's of 92 kDa and 94 kDa.

实验方案

Product Specific Protocols
WB protocol for NLGN4X antibody 27278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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