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验证数据展示

  • WB analysis of mouse kidney using 16699-1-AP (same clone as 16699-1-PBS)

    WB analysis of mouse kidney using 16699-1-AP (same clone as 16699-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16699-1-AP (NKD2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16699-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 16699-1-AP (same clone as 16699-1-PBS)

    WB analysis of HEK-293 using 16699-1-AP (same clone as 16699-1-PBS)

    WB result of NKD2 antibody (16699-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 16699-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

NKD2 Polyclonal antibody, PBS Only

NKD2 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 16699-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

Naked-2, Naked2, Naked 2, hNkd2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

16699-1-PBS targets NKD2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10094

Product name: Recombinant human Naked2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-311 aa of BC012176

Sequence: MGKLQSKHAAAARKRRESPEGDSFVASAYASGRKGAEEAERRARDKQELPNGDPKEGPFREDQCPLQVALPAEKAEGREHPGQLLSADDGERAANREGPRGPGGQRLNIDALQCDVSVEEDDRQEWTFTLYDFDNCGKVTREDMSSLMHTIYEVVDASVNHSSGSSKTLRVKLTVSPEPSSKRKEGPPAGQDREPTRCRMEGELAEEPRVADRRLSAHVRRPSTDPQPCSERGPYCVDENTERRNHYLDLAGIENYTSRFGPGSQLCEKRSSAPRTHSGDKARGVGLCRELWSQAGHPQWPGPFPSGLVAV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 naked cuticle homolog 2 (Drosophila)
别名 Naked-2, Naked2, Naked 2, hNkd2
计算分子量 50 kDa
观测分子量 59 kDa
GenBank蛋白编号BC012176
基因名称 NKD2
Gene ID (NCBI) 85409
RRIDAB_2151468
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969F2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Naked2 (protein naked cuticle homolog 2), encoded by NKD2 gene, was identified as a cell autonomous antagonist of the canonical Wnt signaling pathway. Naked2 acts as a cargo recognition and targeting protein to ensure proper delivery and fusion of TGF-alpha-containing vesicles to a distinct region at the basolateral surface of polarized epithelial cells (PMID: 17553928). EGFR-independent action of TGF-alpha protects Naked2 from proteasomal degradation, thus ensuring its delivery to the basolateral surface (PMID: 18757723). Myristoylated Naked2 antagonizes Wnt-beta-catenin activity possibly by degrading Dishevelled-1 at the plasma membrane (PMID: 20177058).