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验证数据展示

  • WB analysis using 13884-1-AP (same clone as 13884-1-PBS)

    WB analysis using 13884-1-AP (same clone as 13884-1-PBS)

    Mouse brain total extract lysate and 37°C incubated mouse brain membrane extract lysate were subjected to SDS PAGE followed by western blot with 13884-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13884-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 13884-1-AP (same clone as 13884-1-PBS)

    IHC staining of human colon cancer using 13884-1-AP (same clone as 13884-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13884-1-AP (NKCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13884-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 13884-1-AP (same clone as 13884-1-PBS)

    IHC staining of human colon cancer using 13884-1-AP (same clone as 13884-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13884-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13884-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 13884-1-AP (same clone as 13884-1-PBS)

    IF Staining of A549 using 13884-1-AP (same clone as 13884-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using NKCC1/SLC12A2 antibody (13884-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13884-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 13884-1-AP (same clone as 13884-1-PBS)

    IHC staining of human colon cancer using 13884-1-AP (same clone as 13884-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 13884-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13884-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NKCC1/SLC12A2 Polyclonal antibody, PBS Only

NKCC1/SLC12A2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13884-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

NKCC1,SLC12A2, NKCC1, SLC12A2, Basolateral Na K Cl symporter, Basolateral Na-K-Cl symporter

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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For optimal WB detection with 13884-1-AP, we do not recommend boiling the sample after lysis.

产品信息

13884-1-PBS targets NKCC1/SLC12A2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4991

Product name: Recombinant human SLC12A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 759-1150 aa of BC033003

Sequence: GSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEEDGKTATQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKFYEPC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 12 (sodium/potassium/chloride transporters), member 2
别名 NKCC1,SLC12A2, NKCC1, SLC12A2, Basolateral Na K Cl symporter, Basolateral Na-K-Cl symporter
计算分子量 131 kDa
观测分子量 150-160 kDa
GenBank蛋白编号BC033003
基因名称 NKCC1/SLC12A2
Gene ID (NCBI) 6558
RRIDAB_2188522
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55011
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).

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