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验证数据展示

  • WB analysis using 29761-1-AP (same clone as 29761-1-PBS)

    WB analysis using 29761-1-AP (same clone as 29761-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29761-1-AP (NHE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

  • WB analysis using 29761-1-AP (same clone as 29761-1-PBS)

    WB analysis using 29761-1-AP (same clone as 29761-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29761-1-AP (NHE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 29761-1-AP (same clone as 29761-1-PBS)

    IHC staining of human stomach using 29761-1-AP (same clone as 29761-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 29761-1-AP (NHE1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NHE1/SLC9A1 antibody included
  • IHC staining of human stomach using 29761-1-AP (same clone as 29761-1-PBS)

    IHC staining of human stomach using 29761-1-AP (same clone as 29761-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 29761-1-AP (NHE1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NHE1/SLC9A1 antibody included
  • IF Staining of HeLa using 29761-1-AP (same clone as 29761-1-PBS)

    IF Staining of HeLa using 29761-1-AP (same clone as 29761-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NHE1 antibody (29761-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29761-1-PBS in a different storage buffer formulation.

NHE1 Polyclonal antibody, PBS Only

NHE1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29761-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NHE1/SLC9A1 Ready-To-Use IHC Kit. NHE1/SLC9A1 primary antibody included.

产品信息

29761-1-PBS targets NHE1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31192

Product name: Recombinant human NHE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 656-815 aa of NM_003047

Sequence: ADPYEEAWNQMLLRRQKARQLEQKINNYLTVPAHKLDSPTMSRARIGSDPLAYEPKEDLPVITIDPASPQSPESVDLVNEELKGKVLGLSRDPAKVAEEDEDDDGGIMMRSKETSSPGTDDVFTPAPSDSPSSQRIQRCLSDPGPHPEPGEGEPFFPKGQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 9 (sodium/hydrogen exchanger), member 1
别名
计算分子量 91 kDa
观测分子量90-100 kDa
GenBank蛋白编号NM_003047
基因名称 NHE1
Gene ID (NCBI) 6548
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19634
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NHE1, also named as SLC9A1, encodes an Na+/H+ antiporter that is a membrane-bound enzyme involved in regulating pH of vertebrate cells. NHE1 regulates a number of cell behaviors, including adhesion, shape determination, migration, and proliferation (PMID:11807182). It is inhibited by the non-specific diuretic drug amiloride and activated by growth factors, mitogens, neurotransmitters, tumor promoters, and others (PMID: 2846238). NHE1 is expressed in heart where it contributes to cardiomyocyte pH homeostasis (PMID: 12832126, PMID: 23709596).

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