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NEDD4 Polyclonal antibody

NEDD4 Polyclonal Antibody for WB, IP, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat, monkey and More (1)


WB, IP, IF/ICC, IHC, ELISA and More (1)



Cat No : 21698-1-AP

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KIAA0093, NEDD 4, NEDD4, NEDD4 1, RPF1


Positive WB detected inA549 cells, C6 cells, HeLa cells, HT-1080 cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, COS-7 cells
Positive IP detected inC6 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells


Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


21698-1-AP targets NEDD4 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications WB, IP, IF/ICC, IHC, ELISA Application Description
Cited ApplicationsWB, IP, IF, IHC, CoIP
Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, rat, chicken
Immunogen NEDD4 fusion protein Ag16291 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neural precursor cell expressed, developmentally down-regulated 4
Synonyms KIAA0093, NEDD 4, NEDD4, NEDD4 1, RPF1
Calculated Molecular Weight 1319 aa, 149 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC152452
Gene Symbol NEDD4
Gene ID (NCBI) 4734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 and NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).


Product Specific Protocols
WB protocol for NEDD4 antibody 21698-1-APDownload protocol
IHC protocol for NEDD4 antibody 21698-1-APDownload protocol
IF protocol for NEDD4 antibody 21698-1-APDownload protocol
IP protocol for NEDD4 antibody 21698-1-APDownload protocol
Standard Protocols
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J Exp Clin Cancer Res

PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer

Authors - Liang Xu

Proc Natl Acad Sci U S A

Zbtb7b engages the long noncoding RNA Blnc1 to drive brown and beige fat development and thermogenesis.

Authors - Siming Li


PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex.

Authors - Xiaoxiao Wang

Cell Death Differ

Ubiquitin ligase CHIP regulates OTUD3 stability and suppresses tumour metastasis in lung cancer.

Authors - Pengfei Zhang
humanWB, CoIP

Clin Transl Med

Tumor-derived exosomal miR-19b-3p facilitates M2 macrophage polarization and exosomal LINC00273 secretion to promote lung adenocarcinoma metastasis via Hippo pathway.

Authors - Jing Chen


Xinbao Pill attenuated chronic heart failure by suppressing the ubiquitination of β-adrenergic receptors

Authors - Xiaoyu Tian