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验证数据展示

  • WB analysis of mouse liver using 23855-1-AP

    WB analysis of mouse liver using 23855-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 23855-1-AP (NDUFB5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 23855-1-AP

    IHC staining of human liver cancer using 23855-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23855-1-AP (NDUFB5 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 23855-1-AP

    IHC staining of human liver cancer using 23855-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23855-1-AP (NDUFB5 Antibody) at dilution of 1:200 (under 40x lens).

NDUFB5 Polyclonal antibody

NDUFB5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23855-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA

别名

CI SGDH, Complex I SGDH, NDUFB5, SGDH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, mouse spleen tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

23855-1-AP targets NDUFB5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, rat
免疫原 NDUFB5 fusion protein Ag20907 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa
别名 CI SGDH, Complex I SGDH, NDUFB5, SGDH
计算分子量 189 aa, 22 kDa
观测分子量 16 kDa
GenBank蛋白编号BC005271
基因名称 NDUFB5
Gene ID (NCBI) 4711
RRIDAB_2879342
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDO43674
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NDUFB5 antibody 23855-1-APDownload protocol
IHC protocol for NDUFB5 antibody 23855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proteome Sci

Effect of downregulated citrate synthase on oxidative phosphorylation signaling pathway in HEI-OC1 cells

Authors - Xiaowen Xu
View Article
ratWB

Basic Res Cardiol

Comparison of the stage-dependent mitochondrial changes in response to pressure overload between the diseased right and left ventricle in the rat

Authors - Ling Li
View Article