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Species Reactivity
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验证数据展示

  • WB analysis of mouse liver using 23855-1-AP

    WB analysis of mouse liver using 23855-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 23855-1-AP (NDUFB5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 23855-1-AP

    IHC staining of human liver cancer using 23855-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23855-1-AP (NDUFB5 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 23855-1-AP

    IHC staining of human liver cancer using 23855-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23855-1-AP (NDUFB5 Antibody) at dilution of 1:200 (under 40x lens).

NDUFB5 Polyclonal antibody

NDUFB5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23855-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA

别名

CI SGDH, Complex I SGDH, NDUFB5, SGDH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, mouse spleen tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

23855-1-AP targets NDUFB5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, rat
免疫原

CatNo: Ag20907

Product name: Recombinant human NDUFB5 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-189 aa of BC005271

Sequence: MAAMSLLRRVSVTAVAALSGRPLGTRLGFGGFLTRGFPKAAAPVRHSGDHGKRLFVIRPSRFYDRRFLKLLRFYIALTGIPVAIFITLVNVFIGQAELAEIPEGYVPEHWEYYKHPISRWIARNFYDSPEKIYERTMAVLQIEAEKAELRVKELEVRKLMHVRGDGPWYYYETIDKELIDHSPKATPDN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa
别名 CI SGDH, Complex I SGDH, NDUFB5, SGDH
计算分子量 189 aa, 22 kDa
观测分子量 16 kDa
GenBank蛋白编号BC005271
基因名称 NDUFB5
Gene ID (NCBI) 4711
RRIDAB_2879342
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDO43674
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NDUFB5 antibody 23855-1-APDownload protocol
IHC protocol for NDUFB5 antibody 23855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proteome Sci

Effect of downregulated citrate synthase on oxidative phosphorylation signaling pathway in HEI-OC1 cells

Authors - Xiaowen Xu
View Article
ratWB

Basic Res Cardiol

Comparison of the stage-dependent mitochondrial changes in response to pressure overload between the diseased right and left ventricle in the rat

Authors - Ling Li
View Article