验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse spleen tissue, Jurkat cells, K-562 cells, MCF-7 cells, mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
23855-1-AP targets NDUFB5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag20907 Product name: Recombinant human NDUFB5 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-189 aa of BC005271 Sequence: MAAMSLLRRVSVTAVAALSGRPLGTRLGFGGFLTRGFPKAAAPVRHSGDHGKRLFVIRPSRFYDRRFLKLLRFYIALTGIPVAIFITLVNVFIGQAELAEIPEGYVPEHWEYYKHPISRWIARNFYDSPEKIYERTMAVLQIEAEKAELRVKELEVRKLMHVRGDGPWYYYETIDKELIDHSPKATPDN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa |
| 别名 | CI SGDH, CI-SGDH, Complex I SGDH, Complex I-SGDH, NADH-ubiquinone oxidoreductase SGDH subunit |
| 计算分子量 | 189 aa, 22 kDa |
| 观测分子量 | 16 kDa |
| GenBank蛋白编号 | BC005271 |
| 基因名称 | NDUFB5 |
| Gene ID (NCBI) | 4711 |
| RRID | AB_2879342 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | O43674 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDUFB5 (NADH: Ubiquinone Oxidoreductase Subunit B5) is a 16 kDa accessory subunit of mitochondrial respiratory chain Complex I (NADH: ubiquinone oxidoreductase) in humans. NDUFB5 is a non-catalytic accessory subunit of mitochondrial Complex I essential for proper assembly and function of the respiratory chain. It is ubiquitously expressed, highly conserved across species, and anchored in the mitochondrial inner membrane. While not directly involved in catalysis, it plays a structural and regulatory role in Complex I integrity, and its dysfunction may contribute to mitochondrial diseases and certain cancers.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for NDUFB5 antibody 23855-1-AP | Download protocol |
| WB protocol for NDUFB5 antibody 23855-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proteome Sci Effect of downregulated citrate synthase on oxidative phosphorylation signaling pathway in HEI-OC1 cells | ||
Basic Res Cardiol Comparison of the stage-dependent mitochondrial changes in response to pressure overload between the diseased right and left ventricle in the rat |
