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Host
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Species Reactivity
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 28447-1-AP (same clone as 28447-1-PBS)

    WB analysis using 28447-1-AP (same clone as 28447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28447-1-AP (NCX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28447-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 28447-1-AP (same clone as 28447-1-PBS)

    IHC staining of human kidney using 28447-1-AP (same clone as 28447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28447-1-AP (NCX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28447-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SLC8A1 antibody included
  • WB analysis using 28447-1-AP (same clone as 28447-1-PBS)

    WB analysis using 28447-1-AP (same clone as 28447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28447-1-AP (NCX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28447-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 28447-1-AP (same clone as 28447-1-PBS)

    IHC staining of human kidney using 28447-1-AP (same clone as 28447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28447-1-AP (NCX1 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28447-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SLC8A1 antibody included

NCX1 Polyclonal antibody, PBS Only

NCX1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28447-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Na(+)/Ca(2+)-exchange protein 1, SLC8A1, Solute carrier family 8 member 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SLC8A1 Ready-To-Use IHC Kit. SLC8A1 primary antibody included.

产品信息

28447-1-PBS targets NCX1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NCX1 fusion protein Ag28952 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 8 (sodium/calcium exchanger), member 1
别名 Na(+)/Ca(2+)-exchange protein 1, SLC8A1, Solute carrier family 8 member 1
计算分子量 109 kDa
观测分子量70 kDa
GenBank蛋白编号NM_021097
基因名称 NCX1
Gene ID (NCBI) 6546
RRIDAB_2918167
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP32418
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC8A1, also known as NCX1, belongs to the SLC (Solute Carrier) family and the CaCA (Calcium/Cation Antiporter) superfamily. NCX1 is a bidirectional ion exchanger that can exchange sodium and calcium ions between the inside and outside of cells in a 1:3 ratio (1 Ca2+ ion and 3 Na+ ions). Under physiological conditions, NCX1 mainly transports Ca2+ from the cell interior to the exterior through the forward transport mode to maintain the low calcium level inside the cell. However, under certain special conditions, such as ischemia-reperfusion injury and stroke, the reverse transport mode of NCX1 is activated, leading to the influx of Ca2+ into the cell, which may cause cell damage (PMID: 21289289, 30686898).