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Host
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Species Reactivity
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验证数据展示

  • WB analysis using 29587-1-AP (same clone as 29587-1-PBS)

    WB analysis using 29587-1-AP (same clone as 29587-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29587-1-AP (NCOA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 29587-1-AP (same clone as 29587-1-PBS)

    IHC staining of human lung cancer using 29587-1-AP (same clone as 29587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29587-1-AP (NCOA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 29587-1-AP (same clone as 29587-1-PBS)

    IF Staining of HeLa using 29587-1-AP (same clone as 29587-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NCOA3 antibody (29587-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

  • WB analysis using 29587-1-AP (same clone as 29587-1-PBS)

    WB analysis using 29587-1-AP (same clone as 29587-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29587-1-AP (NCOA3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29587-1-PBS in a different storage buffer formulation.

NCOA3 Polyclonal antibody, PBS Only

NCOA3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29587-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

AIB1, SRC3, ACTR, AIB 1, AIB-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

29587-1-PBS targets NCOA3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 NCOA3 fusion protein Ag30633 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nuclear receptor coactivator 3
别名 AIB1, SRC3, ACTR, AIB 1, AIB-1
计算分子量 1424 aa, 155 kDa
观测分子量150-160 kDa
GenBank蛋白编号BC119001
基因名称 NCOA3
Gene ID (NCBI) 8202
RRIDAB_2918328
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6Q9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NCOA3, also named as AIB1, SRC3 and TRAM1, belongs to the SRC/p160 nuclear receptor coactivator family. NCOA3 is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. NOCA3 plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). NCOA3 also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.