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Neurochondrin Polyclonal antibody

Neurochondrin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 13187-1-AP

Print datasheet

Synonyms

KIAA0607, NCDN, Neurochondrin

验证数据展示

  • WB analysis using 13187-1-AP

    WB analysis using 13187-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13187-1-AP (Neurochondrin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13187-1-AP

    IHC staining of mouse brain using 13187-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13187-1-AP (Neurochondrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13187-1-AP

    IHC staining of mouse brain using 13187-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13187-1-AP (Neurochondrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13187-1-AP targets Neurochondrin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Neurochondrin fusion protein Ag3859 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neurochondrin
Synonyms KIAA0607, NCDN, Neurochondrin
Calculated Molecular Weight 729 aa, 79 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC024592
Gene Symbol NCDN
Gene ID (NCBI) 23154
RRIDAB_2877919
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBB6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Neurochondrin antibody 13187-1-APDownload protocol
IHC protocol for Neurochondrin antibody 13187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

J Cell Sci

Neurochondrin interacts with the SMN protein suggesting a novel mechanism for spinal muscular atrophy pathology.

Authors - Luke W Thompson
View Article