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验证数据展示

  • WB analysis of fetal human brain using 60238-1-Ig (same clone as 60238-1-PBS)

    WB analysis of fetal human brain using 60238-1-Ig (same clone as 60238-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60238-1-Ig (NCAM1/CD56 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 60238-1-Ig (same clone as 60238-1-PBS)

    IHC staining of human lung cancer using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NCAM1/CD56 antibody included
  • IHC staining of human lung cancer using 60238-1-Ig (same clone as 60238-1-PBS)

    IHC staining of human lung cancer using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NCAM1/CD56 antibody included
  • IF Staining of human colon using 60238-1-Ig (same clone as 60238-1-PBS)

    IF Staining of human colon using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using NCAM1/CD56 antibody (60238-1-Ig, Clone: 1E8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 60238-1-Ig (same clone as 60238-1-PBS)

    IF Staining of SH-SY5Y using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using NCAM1/CD56 antibody (60238-1-Ig, Clone: 1E8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 60238-1-Ig (same clone as 60238-1-PBS)

    IHC staining of human colon using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NCAM1/CD56 antibody included
  • IF Staining of human tonsillitis using 60238-1-Ig (same clone as 60238-1-PBS)

    IF Staining of human tonsillitis using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using NCAM1/CD56 antibody (60238-1-Ig, Clone: 1E8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

NCAM1/CD56 Monoclonal antibody, PBS Only

NCAM1/CD56 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 60238-1-PBS

产品说明书

CloneNo. 1E8C9

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

CD56, NCAM1, 1E8C9, MSK39, N CAM 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NCAM1/CD56 Ready-To-Use IHC Kit. NCAM1/CD56 primary antibody included.

产品信息

60238-1-PBS targets NCAM1/CD56 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, rat, pig
免疫原

CatNo: Ag5732

Product name: Recombinant human NCAM1/CD56 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 30-352 aa of BC047244

Sequence: EISVGESKFFLCQVAGDAKDKDISWFSPNGEKLTPNQQRISVVWNDDSSSTLTIYNANIDDAGIYKCVVTGEDGSESEATVNVKIFQKLMFKNAPTPQEFREGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTIQARQNIVNATANLGQSVTLVCDAEGFPEPTMSWTKDGEQIEQEEDDEKYIFSDDSSQLTIKKVDKNDEAEYICIAENKAGEQDATIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEE

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 neural cell adhesion molecule 1
别名 CD56, NCAM1, 1E8C9, MSK39, N CAM 1
计算分子量 95 kDa
观测分子量 140 kDa
GenBank蛋白编号BC047244
基因名称 NCAM1
Gene ID (NCBI) 4684
ENSEMBL Gene IDENSG00000149294
RRIDAB_2881361
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP13591
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).

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