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NCAM1/CD56 Monoclonal antibody, PBS Only

NCAM1/CD56 Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

1E8C9

Cat No : 60238-1-PBS

Print datasheet

Synonyms

CD56, MSK39, N CAM 1, NCAM, NCAM 1, NCAM1, NCAM1/CD56

验证数据展示

  • WB analysis of fetal human brain using 60238-1-Ig (same clone as 60238-1-PBS)

    WB analysis of fetal human brain using 60238-1-Ig (same clone as 60238-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60238-1-Ig (NCAM1/CD56 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 60238-1-Ig (same clone as 60238-1-PBS)

    IHC staining of human lung cancer using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD56/NCAM1 antibody included
  • IHC staining of human lung cancer using 60238-1-Ig (same clone as 60238-1-PBS)

    IHC staining of human lung cancer using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD56/NCAM1 antibody included
  • IF Staining of SH-SY5Y using 60238-1-Ig (same clone as 60238-1-PBS)

    IF Staining of SH-SY5Y using 60238-1-Ig (same clone as 60238-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using NCAM1/CD56 antibody (60238-1-Ig, Clone: 1E8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60238-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD56/NCAM1 Ready-To-Use IHC Kit. CD56/NCAM1 primary antibody included.

产品信息

60238-1-PBS targets NCAM1/CD56 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications WB,IHC,IF,ELISA
Tested Reactivity human, mouse, pig, rat
Immunogen NCAM1/CD56 fusion protein Ag5732 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name neural cell adhesion molecule 1
Synonyms CD56, MSK39, N CAM 1, NCAM, NCAM 1, NCAM1, NCAM1/CD56
Calculated Molecular Weight 95 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC047244
Gene Symbol NCAM1
Gene ID (NCBI) 4684
ENSEMBL Gene IDENSG00000149294
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13591
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).