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验证数据展示

  • IHC staining of human liver using 13242-1-AP

    IHC staining of human liver using 13242-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13242-1-AP (NBPF1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 13242-1-AP

    IP experiment of HeLa using 13242-1-AP

    IP result of anti-NBPF1 (IP:13242-1-AP, 4ug; Detection:13242-1-AP 1:300) with HeLa cells lysate 1760 ug.

  • IF Staining of Jurkat using 13242-1-AP

    IF Staining of Jurkat using 13242-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using NBPF1 antibody (13242-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

NBPF1 Polyclonal antibody

NBPF1 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Cat No. 13242-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, IP, ELISA

别名

Neuroblastoma breakpoint family member 1, KIAA1693

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13242-1-AP targets NBPF1 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 NBPF1 fusion protein Ag4096 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neuroblastoma breakpoint family, member 1
别名 Neuroblastoma breakpoint family member 1, KIAA1693
计算分子量 1213 aa, 139 kDa
观测分子量139 kDa
GenBank蛋白编号BC034418
基因名称 NBPF1
Gene ID (NCBI) 55672
RRIDAB_3669156
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3BBV0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NBPF1 (Neuroblastoma Breakpoint Family, member 1) was originally identified in a neuroblastoma patient on the basis of its disruption by a chromosomal translocation t(1;17)

实验方案

Product Specific Protocols
IHC protocol for NBPF1 antibody 13242-1-APDownload protocol
IF protocol for NBPF1 antibody 13242-1-APDownload protocol
IP protocol for NBPF1 antibody 13242-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols