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验证数据展示

  • WB analysis of A375 using 14394-1-AP

    WB analysis of A375 using 14394-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 14394-1-AP (NAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 14394-1-AP

    IHC staining of human breast cancer using 14394-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14394-1-AP (NAT1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 14394-1-AP

    IHC staining of human breast cancer using 14394-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14394-1-AP (NAT1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A375 using 14394-1-AP

    IF Staining of A375 using 14394-1-AP

    Immunofluorescent analysis of A375 cells using 14394-1-AP (NAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

NAT1 Polyclonal antibody

NAT1 Polyclonal Antibody for WB, IF, IHC, ELISA
Cat No. 14394-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF, IHC, ELISA

别名

AAC1, Arylamide acetylase 1, MNAT, N acetyltransferase type 1, NAT 1, NAT1, NATI

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14394-1-AP targets NAT1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 NAT1 fusion protein Ag5885 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-acetyltransferase 1 (arylamine N-acetyltransferase)
别名 AAC1, Arylamide acetylase 1, MNAT, N acetyltransferase type 1, NAT 1, NAT1, NATI
计算分子量 34 kDa
观测分子量 32-34 kDa
GenBank蛋白编号BC047666
基因名称 NAT1
Gene ID (NCBI) 9
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP18440
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.