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验证数据展示

  • WB analysis of A375 using 14394-1-AP

    WB analysis of A375 using 14394-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 14394-1-AP (NAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 14394-1-AP

    IHC staining of human breast cancer using 14394-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14394-1-AP (NAT1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 14394-1-AP

    IHC staining of human breast cancer using 14394-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14394-1-AP (NAT1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A375 using 14394-1-AP

    IF Staining of A375 using 14394-1-AP

    Immunofluorescent analysis of A375 cells using 14394-1-AP (NAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

NAT1 Polyclonal antibody

NAT1 Polyclonal Antibody for WB, IF, IHC, ELISA
Cat No. 14394-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF, IHC, ELISA

别名

AAC1, Arylamide acetylase 1, MNAT, N acetyltransferase type 1, NAT 1, NAT1, NATI

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA375 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14394-1-AP targets NAT1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5885

Product name: Recombinant human NAT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-290 aa of BC047666

Sequence: MDIEAYLERIGYKKSRNKLDLETLTDILQHQIRAVPFENLNIHCGDAMDLGLEAIFDQVVRRNRGGWCLQVNHLLYWALTTIGFETTMLGGYVYSTPAKKYSTGMIHLLLQVTIDGRNYIVDAGFGRSYQMWQPLELISGKDQPQVPCVFRLTEENGFWYLDQIRREQYIPNEEFLHSDLLEDSKYRKIYSFTLKPRTIEDFESMNTYLQTSPSSVFTSKSFCSLQTPDGVHCLVGFTLTHRRFNYKDNTDLIEFKTLSEEEIEKVLKNIFNISLQRKLVPKHGDRFFTI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-acetyltransferase 1 (arylamine N-acetyltransferase)
别名 AAC1, Arylamide acetylase 1, MNAT, N acetyltransferase type 1, NAT 1, NAT1, NATI
计算分子量 34 kDa
观测分子量 32-34 kDa
GenBank蛋白编号BC047666
基因名称 NAT1
Gene ID (NCBI) 9
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP18440
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.