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验证数据展示

  • Single Cell Sequencing experiment G60200-1-5C on Resting PBMC

    Single Cell Sequencing experiment G60200-1-5C on Resting PBMC

    G60200-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G60200-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G60200-1-5C on PBMC treated with 4hr LPS + TI

    G60200-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

MultiPro® 5CFLX Anti-Human ZAP70 (1A11G9)

ZAP70 Monoclonal Antibody for Single Cell (Intra)
Cat No. G60200-1-5C

产品说明书

CloneNo. 1A11G9

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

Single Cell (Intra)

别名

70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70

缓冲液配方:  PBS, Azide
PBS, Azide
偶联物:  5CFLX
规格价格库存

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G60200-1-5C targets ZAP70 in Single Cell (Intra) applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra) Application Description
经测试反应性 Human
免疫原

CatNo: Ag7943

Product name: Recombinant human ZAP70 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 149-493 aa of BC039039

Sequence: STLTHPQRRIDTLNSDGYTPEPARITSPDKPRPMPMDTSVYESPYSDPEELKDKKLFLKRDNLLIADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQGTEKADTEEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKREEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALMSDCWIYKWEDRPDFLTVEQRMRACYYSLASKVEGPPGSTQKAEAACA

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human ZAP70 (1A11G9)
别名 70 kDa zeta associated protein, SRK, STD, Syk related tyrosine kinase, Tyrosine protein kinase ZAP 70, TZK, ZAP 70, ZAP70
计算分子量 70 kDa
GenBank蛋白编号BC039039
基因名称 ZAP70
Gene ID (NCBI) 7535
ENSEMBL Gene IDENSG00000115085
RRIDAB_3673899
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGCTCTACCGCGCGACGCCCATATAAGAAA
条形码序列CTCTACCGCGCGACG
形式Liquid
UNIPROT IDP43403
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3.
储存条件2-8°C Stable for one year after shipment.

背景介绍

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol
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