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验证数据展示

  • Single Cell Sequencing experiment G80084-1-5C on Resting PBMC

    Single Cell Sequencing experiment G80084-1-5C on Resting PBMC

    G80084-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G80084-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G80084-1-5C on PBMC treated with 4hr LPS + TI

    G80084-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

MultiPro® 5CFLX Anti-Human Phospho-Beta Catenin (Ser675) (6D6)

Phospho-Beta Catenin (Ser675) Recombinant Antibody for Single Cell (Intra)
Cat No. G80084-1-5C

产品说明书

CloneNo. 6D6

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

Single Cell (Intra)

别名

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  5CFLX
规格价格库存

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G80084-1-5C targets Phospho-Beta Catenin (Ser675) in Single Cell (Intra) applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra) Application Description
经测试反应性 Human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Oligo Conjugate
产品类型 Recombinant
全称 MultiPro® 5CFLX Anti-Human Phospho-Beta Catenin (Ser675) (6D6)
别名 b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286
计算分子量 781 aa, 86 kDa
GenBank蛋白编号BC058926
基因名称 Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_3673975
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGATATCTTAATCCGAACCCATATAAGAAA
条形码序列ATATCTTAATCCGAA
形式Liquid
UNIPROT IDP35222
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3.
储存条件2-8°C Stable for one year after shipment.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). PKA was shown to phosphorylate β-catenin at Ser675. Phosphorylation at Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol