验证数据展示
经过测试的应用
| Positive Single Cell (Intra) detected in | 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product. | 
推荐稀释比
| 应用 | 推荐稀释比 | 
|---|---|
| SINGLE CELL (INTRA) | <0.5ug/test | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
产品信息
G67722-1-5C targets GATA2 in Single Cell (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | Single Cell (Intra) Application Description | 
| 经测试反应性 | Human | 
| 免疫原 | 
                                             CatNo: Ag1557 Product name: Recombinant human GATA2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 168-467 aa of BC018988 Sequence: SHLFGFPPTPPKEVSPDPSTTGAASPASSSAGGSAARGEDKDGVKYQVSLTESMKMESGSPLRPGLATMGTQPATHHPIPTYPSYVPAAAHDYSSGLFHPGGFLGGPASSFTPKQRSKARSCSEGRECVNCGATATPLWRRDGTGHYLCNACGLYHKMNGQNRPLIKPKRRLTTTTTLWRRNANGDPVCNACGLYYKLHNVNRPLTMKKEGIQTRNRKMSNKSKKSKKGAECFEELSKCMQEKSSPFSAAALAGHMAPVGHLPPFSHSGHILPTPTPIHPSSSLSFGHPHPSSMVTAMG 种属同源性预测 | 
                                    
| 宿主/亚型 | Mouse / IgG1 | 
| 抗体类别 | Oligo Conjugate | 
| 产品类型 | Monoclonal | 
| 全称 | MultiPro® 5CFLX Anti-Human GATA2 (2B5A1) | 
| 别名 | FLJ45948, GATA binding protein 2, GATA2, NFE1B | 
| GenBank蛋白编号 | BC018988 | 
| 基因名称 | GATA2 | 
| Gene ID (NCBI) | 2624 | 
| ENSEMBL Gene ID | ENSG00000179348 | 
| RRID | AB_3673967 | 
| 偶联类型 | 5CFLX | 
| 完整寡核苷酸序列 | CGGAGATGTGTATAAGAGACAGTCACGCCGTCACGAGCCCATATAAGAAA | 
| 条形码序列 | TCACGCCGTCACGAG | 
| 形式 | Liquid | 
| UNIPROT ID | P23769 | 
| 储存缓冲液 | PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3. | 
| 储存条件 | 2-8°C Stable for one year after shipment. | 
背景介绍
GATA2 is a member of GATA family of transcription factors that contains zinc fingers in DNA binding domain. GATA2 acts as a transcriptinal activator that regulates endothelin-1 expression in endothelial cells. Together with GATA3, GATA2 regulates adipocyte differentiation through molecular control of the preadpocyte-adipocyte transition. It also affect the activity of Rho inhibitor p190RhoGAP that controls capillary network formation in vitro and retinal angiogenesis in vivo.
Protocols
| Our current MultiPro staining protocol for the cocktail | Download protocol | 
| 10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdf | Download protocol | 

