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验证数据展示

  • Single Cell Sequencing experiment G66180-1-5C on Resting PBMC

    Single Cell Sequencing experiment G66180-1-5C on Resting PBMC

    G66180-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66180-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66180-1-5C on PBMC treated with 4hr LPS + TI

    G66180-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

MultiPro® 5CFLX Anti-Human CD71 (3C11F11)

CD71 Monoclonal Antibody for Single Cell (Intra)
Cat No. G66180-1-5C

产品说明书

CloneNo. 3C11F11

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

Single Cell (Intra)

别名

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

缓冲液配方:  PBS, Azide
PBS, Azide
偶联物:  5CFLX
规格价格库存

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CD71 Ready-To-Use IHC Kit. CD71 primary antibody included.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G66180-1-5C targets CD71 in Single Cell (Intra) applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra) Application Description
经测试反应性 Human
免疫原

CatNo: Ag21612

Product name: Recombinant human CD71 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 513-757 aa of BC001188

Sequence: VKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDID

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human CD71 (3C11F11)
别名 CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR
计算分子量 85 kDa
GenBank蛋白编号BC001188
基因名称 CD71
Gene ID (NCBI) 7037
ENSEMBL Gene IDENSG00000072274
RRIDAB_3673944
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGAACCACACTTACGTTCCCATATAAGAAA
条形码序列AACCACACTTACGTT
形式Liquid
UNIPROT IDP02786
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3.
储存条件2-8°C Stable for one year after shipment.

背景介绍

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol
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