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验证数据展示

  • Single Cell Sequencing experiment G65163-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65163-1-5C on Resting PBMC

    G65163-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65163-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65163-1-5C on PBMC treated with 4hr LPS + TI

    G65163-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

MultiPro® 5CFLX Anti-Human CD42b (AK2)

CD42b Monoclonal Antibody for Single Cell (Intra)
Cat No. G65163-1-5C

产品说明书

CloneNo. AK2

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

Single Cell (Intra)

别名

AK2, BSS, CD42B, CD42b alpha, GP1B, GP1BA, GPIb alpha, GPIbα

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65163-1-5C targets CD42b in Single Cell (Intra) applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra) Application Description
经测试反应性 Human
免疫原 Human platelets 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human CD42b (AK2)
别名 AK2, BSS, CD42B, CD42b alpha, GP1B, GP1BA, GPIb alpha, GPIbα
计算分子量 626 aa, 69 kDa
GenBank蛋白编号BC027955
基因名称 CD42b
Gene ID (NCBI) 2811
ENSEMBL Gene IDENSG00000185245
RRIDAB_3673922
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGCCGTCCATCGGACGCCCCATATAAGAAA
条形码序列CCGTCCATCGGACGC
形式 Liquid
UNIPROT IDP07359
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3
储存条件2-8°C Stable for one year after shipment.

背景介绍

CD42b, also known as platelet glycoprotein Ib alpha chain (GPIb alpha), is a type I transmembrane glycoprotein of 135-145 kDa (PMID: 2656709). It is expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are linked by a disulphide bond to form GPIb (PMID: 7660135). GPIb forms a noncovalent complex with CD42a (GPIX) and CD42d (GPV) and acts as a receptor for von Willebrand factor (vWF) and thrombin (PMID: 7660135; 3759960). The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to blood vessels. Defects in the expression of CD42b result in Bernard-Soulier syndromes and platelet-type von Willebrand disease (PMID: 9371310).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol