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验证数据展示

  • Single Cell Sequencing experiment G65133-1-5C on Resting PBMC

    Single Cell Sequencing experiment G65133-1-5C on Resting PBMC

    G65133-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G65133-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G65133-1-5C on PBMC treated with 4hr LPS + TI

    G65133-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

MultiPro® 5CFLX Anti-Human CD3 (OKT3)

CD3 Monoclonal Antibody for Single Cell (Intra)
Cat No. G65133-1-5C

产品说明书

CloneNo. OKT3

宿主/亚型

Mouse / IgG2a, kappa

种属反应性

Human

应用

Single Cell (Intra)

别名

CD3, CD3E, T3E, TCRE

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.

推荐稀释比

应用推荐稀释比
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G65133-1-5C targets CD3 in Single Cell (Intra) applications and shows reactivity with Human samples.

经测试应用 Single Cell (Intra) Application Description
经测试反应性 Human
免疫原 Human T cells 种属同源性预测
宿主/亚型 Mouse / IgG2a, kappa
抗体类别 Oligo Conjugate
产品类型 Monoclonal
全称 MultiPro® 5CFLX Anti-Human CD3 (OKT3)
别名 CD3, CD3E, T3E, TCRE
计算分子量 207 aa, 23 kDa
GenBank蛋白编号BC049847
基因名称 CD3
Gene ID (NCBI) 916
ENSEMBL Gene IDENSG00000198851
RRIDAB_3673918
偶联类型 5CFLX
完整寡核苷酸序列CGGAGATGTGTATAAGAGACAGTCGGATAAGACCTTCCCCATATAAGAAA
条形码序列TCGGATAAGACCTTC
形式 Liquid
UNIPROT IDP07766
储存缓冲液 PBS with 1mM EDTA and 0.09% sodium azide , pH 7.3
储存条件2-8°C Stable for one year after shipment.

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol