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Recombinant Mouse gp130/IL6ST protein (rFc Tag)(HPLC verified)

种属

Mouse

纯度

>90 %, SDS-PAGE
>90 %, SEC-HPLC

标签

rFc Tag

生物活性

未测试

Cat no : Eg1631

Print datasheet

Synonyms

CD130, gp130, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6RB

验证数据展示

  • Purity of Recombinant Mouse gp130 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse gp130 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse gp130/IL6ST was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse gp130/IL6ST was greater than 90% as determined by SEC-HPLC.

产品信息

纯度 >90 %, SDS-PAGE
>90 %, SEC-HPLC
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Mouse gp130 protein Gln23-Glu617 (Accession# Q00560) with a rabbit IgG Fc tag at the C-terminus.
基因ID 16195
蛋白编号 Q00560
预测分子量 92.9 kDa
SDS-PAGE 90-125 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC). These cytokines signal through the gp130/Jak/STAT pathway. Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells.

参考文献:

1. T Taga, et al. (1997) Annu Rev Immunol. 15:797-819.
2. J S Silver, et al. (2010) J Leukoc Biol. 88(6):1145-56.
3. P C Heinrich, et al. (1998) Biochem J. 334 ( Pt 2):297-314.
4. F A Montero-Julian, et al. (1997) Clin Cancer Res. 3(8):1443-51.