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Recombinant Mouse Tie-2/CD202b protein (rFc Tag)

种属

Mouse

纯度

>90 %, SDS-PAGE

标签

rFc Tag

生物活性

未测试

Cat no : Eg1514

Print datasheet

Synonyms

Angiopoietin-1 receptor, CD202b, EC:2.7.10.1, Endothelial tyrosine kinase, HYK

验证数据展示

  • Purity of Recombinant Mouse Tie-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Tie-2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Mouse Tie-2 protein Ala23-Lys744 (Accession# Q02858) with a rabbit IgG Fc tag at the C-terminus.
基因ID 21687
蛋白编号 Q02858
预测分子量 106.9 kDa
SDS-PAGE 120-140 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Tie-2, also known as CD202b or TEK, is a receptor tyrosine kinase (RTK) specifically expressed in endothelial cells. It plays a crucial role in angiogenesis, forming new blood vessels, and maintaining vascular stability. TIE-2 is a tyrosine kinase receptor expressed mainly by endothelial cells, cancer cells, and some immune cells like monocytes. Tie-2 is a member of the Tie receptor family, characterized by the presence of immunoglobulin and epidermal growth factor (EGF) homology domains. Tie-2 is selectively expressed in endothelial cells and gene deficiency of Tie-2 leads to embryonic lethality due to abnormal vascular development. Ang-1, which is secreted from perivascular cells, has been identified as the major ligand for Tie-2. Binding of Ang-1 to Tie-2 elicits different downstream signaling pathways mainly regulated by Akt, which induces pro-angiogenic responses including the promotion of endothelial cell migration, tube formation and survival.

参考文献:

1. Dumont DJ. et al. (1992) Oncogene. 7(8):1471-80.
2. Duran CL. et al. (2021) Cancers (Basel). 13(22):5730.
3. Sato TN. et al. (1995) Nature. 376(6535):70-4.
4. Isidori AM. et al. (2016) J Endocrinol Invest. 39(11):1235-1246.
5. Fukuhara S. et al. (2010) Histol Histopathol. 25(3):387-96.