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Recombinant Mouse CD146/MCAM protein (rFc Tag) (HPLC verified)

种属

Mouse

纯度

>90 %, SDS-PAGE
>90 %, SEC-HPLC

标签

rFc Tag

生物活性

未测试

Cat no : Eg2667

Print datasheet

Synonyms

CD146, Cell surface glycoprotein MUC18, Gicerin, Mcam, Melanoma cell adhesion molecule

验证数据展示

  • Purity of Recombinant Mouse CD146 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CD146 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse CD146 was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse CD146 was greater than 90% as determined by SEC-HPLC.

产品信息

纯度 >90 %, SDS-PAGE
>90 %, SEC-HPLC
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Mouse CD146 protein Val24-Val563 (Accession# Q8R2Y2-1) with a rabbit IgG Fc tag at the C-terminus.
基因ID 84004
蛋白编号 Q8R2Y2-1
预测分子量 85.8 kDa
SDS-PAGE 100-110 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily. Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells.

参考文献:

1. Sers C. et al. (1993). Proc Natl Acad Sci U S A.15;90(18):8514-8. 
2. Keller T. et al. (2015). PLoS One. 20;10(5):e0127169. 
3. Bardin N. et al. (1998). FEBS Lett. 2;421(1):12-4. 
4. Bardin N. et al. (2009). Arterioscler Thromb Vasc Biol.29(5):746-53. 
5. Bardin N. et al. (2006). Inflamm Bowel Dis.12(1):16-21. 
6. Bardin N. et al. (2003). Thromb Haemost. 90(5):915-20.