Speedy™ Mouse LIX/CXCL5 One-Step ELISA Kit
Sensitivity
0.5 pg/mL
Range
3.13-200 pg/mL
Reactivity
Mouse
Cat no : SE60078
Synonyms
C-X-C motif chemokine 5, Cxcl5, Cytokine LIX, GCP-2(1-78), GCP-2(9-78)
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse LIX/CXCL5 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernate, Urine, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 3.13-200 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20311 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 101% | 87%-113% |
| Cell culture supernate | 101% | 96%-110% |
| Urine | 96% | 84%-117% |
| Tissue lysate | 107% | 76%-120% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 98.6 | 1.6 | 1.6 |
| 2 | 8 | 26.2 | 0.8 | 3.1 |
| 3 | 8 | 14.7 | 0.3 | 2.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 95.7 | 4.0 | 4.2 |
| 2 | 16 | 26.2 | 0.9 | 3.4 |
| 3 | 16 | 14.4 | 0.4 | 2.8 |
Background Information
LPS-induced CXC chemokine (LIX) is a murine chemokine similar to two human chemokines, ENA-78 (CXCL5) and GCP-2 (CXCL6). Mouse LIX can be produced by many immune cells, such as macrophages, eosinophils , as well as non-immune cells including mesothelial cells, and fibroblasts. Increased serum LIX has been observed in obesity and insulin resistance in mice and humans and is associated with complications of diabetes including nephropathy and atherosclerosis. It is mainly produced when induced by LPS, IL-17 and/or TNF-α. According to the previous studies, cancer cell autonomous secretion of LIX as sufficient to drive infiltration of mature, protumorigenic neutrophils in a mouse model of non-small cell lung cancer (NSCLC).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C-X-C motif chemokine 5, Cxcl5, Cytokine LIX, GCP-2(1-78), GCP-2(9-78) |
