Recombinant Mouse Cfd protein (rFc Tag)
种属
Mouse
纯度
>90 %, SDS-PAGE
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Mouse Cfd protein Gln21-Ser259 (Accession# P03953-1) with a rabbit IgG Fc tag at the C-terminus. |
| 基因ID | 11537 |
| 蛋白编号 | P03953-1 |
| 预测分子量 | 52.1 kDa |
| SDS-PAGE | 60-75 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
Cfd is a crucial component of the complement system, playing a significant role in host defense and immune regulation. The protein encoded by the Cfd gene is a member of the chymotrypsin family of serine proteases. It plays an essential role in host defense as the rate-limiting enzyme in the alternative pathway of complement activation. Complement factor D activates a convertase (C3bBb) responsible for cleavage of the complement protein C3, which leads to the activation of terminal complement component C5-9 to form the membrane attack complex on microbial or cellular surfaces. Cfd knockout mice display impaired alternative pathway activation of the complement system and increased susceptibility to pneumococcal infection. These mice have a defect in the activation of the alternative pathway, leading to a reduced ability to clear pathogens. Mouse models have been used to study the function of Cfd in various disease states, and its human ortholog CFD is a promising candidate for the development of therapeutic strategies for metabolic and inflammatory diseases.
参考文献:
1. Wang L, Gao P, et al. (2023) J Cachexia Sarcopenia Muscle. 14(5):2152-2167. 2. Cho M, Hwang JS, et al. (2024) Int J Mol Sci. 25(18):9877. 3. Ito S, Hashimoto H, et al. (2022) Nat Commun. 13(1):5409. 4. Ma L, Gilani A, et al. (2024) JCI Insight. 9(11):e178925.
