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Recombinant Mouse CD55 protein (rFc Tag)(HPLC verified)

种属

Mouse

纯度

>90 %, SDS-PAGE
>90 %, SEC-HPLC

标签

rFc Tag

生物活性

未测试

Cat no : Eg2124

Print datasheet

Synonyms

Cd55a, Complement decay-accelerating factor, GPI-anchored, Daf, Daf1, DAF-GPI

验证数据展示

  • Purity of Recombinant Mouse CD55 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CD55 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse CD55 was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse CD55 was greater than 90% as determined by SEC-HPLC.

产品信息

纯度 >90 %, SDS-PAGE
>90 %, SEC-HPLC
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Mouse CD55 protein Asp35-Thr361 (Accession# Q61475) with a rabbit IgG Fc tag at the C-terminus.
基因ID 13136
蛋白编号 Q61475
预测分子量 62.0 kDa
SDS-PAGE 68-90 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

CD55, also known as DAF, is a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein that is widely distributed on blood, stroma, epithelial, and endothelial cells. It can also exist as a soluble form in plasma, urine, saliva, tears, and synovial fluids. CD55 is a complement regulatory protein. It inhibits formation of the C3 convertases through binding to C3b and C4b. It also binds the alternate pathway convertase C3bBb, the classical pathway convertase, and C4b2a to accelerate their decay. CD55 also serves as a receptor for coxsackieviruses B1, B3, and B5 and several enteroviruses. The observed molecular weight of mature CD55 varies between 50 to 100 kDa depending on the cell type. Different sizes of CD55 might be caused by alternative splicing or different glycosylation patterns.

参考文献:

1. J M Bergelson, et al. (1994) Proc Natl Acad Sci U S A. Jun 21;91(13):6245-8.
2. So Hee Dho, et al. (2018) Immune Netw. Feb 20;18(1):e11.
3. D M Lublin 1, J P Atkinson. (1989) Annu Rev Immunol. 7:35-58.
4. Alejandro Ruiz-Argüelles 1, Luis Llorente. (2007) Autoimmun Rev. Jan;6(3):155-61.
5. D R Shafren, et al. (1995) J Virol. Jun;69(6):3873-7.