Mouse CD134/OX40 ELISA Kit
Sensitivity
0.9 pg/mL
Range
39-2500 pg/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
KE10231 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse CD134/OX40 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CD134/OX40. The assay recognizes mouse CD134/OX40. An antibody specific for mouse CD134/OX40 has been pre-coated onto the microwells. The mouse CD134/OX40 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for mouse CD134/OX40 is added to detect the captured mouse CD134/OX40 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse CD134/OX40 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.9 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 22163 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 82% | 79%-84% |
| Cell culture supernatant | 92% | 76%-103% |
| Tissue lysate | 92% | 88%-96% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,262.5 | 54.8 | 4.3 |
| 2 | 8 | 301.1 | 7.6 | 2.5 |
| 3 | 8 | 148.0 | 8.7 | 5.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,313.3 | 82.1 | 6.3 |
| 2 | 16 | 303.2 | 12.1 | 4.0 |
| 3 | 16 | 149.5 | 11.7 | 7.8 |
Background Information
CD134 (OX40) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule. CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmunity. CD134 can interact with TRAF2, TRAF3, and TRAF5.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD134, Tnfrsf4, Ox40, OX40 antigen, OX40L receptor |
