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Recombinant Mouse CD117/c-kit protein (His Tag)

种属

Mouse

纯度

>95 %, SDS-PAGE

标签

His Tag

生物活性

EC50: 2-8 ng/mL

Cat no : Eg0883

Print datasheet

Synonyms

CD117/c-kit, CD117, Kit, c KIT

验证数据展示

  • Purity of Recombinant Mouse CD117 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CD117 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Mouse CD117 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse SCF (His tag) with a linear range of 2-8 ng/mL.

    Immobilized Mouse CD117 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse SCF (His tag) with a linear range of 2-8 ng/mL.

产品信息

纯度 >95 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Immobilized Mouse CD117 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse SCF (His tag) with a linear range of 2-8 ng/mL.
来源 HEK293-derived Mouse CD117 protein Ser25-Pro527 (Accession# P05532-1) with a His tag at the C-terminus.
基因ID 16590
蛋白编号 P05532-1
预测分子量 56.8 kDa
SDS-PAGE 65-85 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit. It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3. CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system.

参考文献:

1.Y Yarden, et al. (1987) EMBO J. 6(11):3341-51.
2.L K Ashman (1999) Int J Biochem Cell Biol. 31(10):1037-51.
3.Johan Lennartsson (2012) Physiol Rev. 92(4):1619-49. 
4.Brittni M Foster, et al. (2018) Biomedicines. 6(1):31.