Speedy™ Mouse C5a One-Step ELISA Kit
Sensitivity
15.1 pg/mL
Range
46.88-3000 pg/mL
Reactivity
Mouse
Cat no : SE60061
Synonyms
C5, C5a anaphylatoxin, Complement C5, Complement C5 alpha chain, Complement C5 alpha' chain
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse C5a One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 15.1 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 15139 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 93% | 90%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,131.1 | 86.8 | 7.7 |
| 2 | 8 | 327.1 | 11.5 | 3.5 |
| 3 | 8 | 188.0 | 16.2 | 8.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,131.3 | 59.8 | 5.3 |
| 2 | 16 | 319.9 | 12.5 | 3.9 |
| 3 | 16 | 182.0 | 15.4 | 8.5 |
Background Information
The complement system is an important effector that bridges the innate and adaptive immune systems. The fifth component of complement, C5, is part of the complement cascade and plays an important role in inflammation and cell killing. It consists of disulfide-linked alpha and beta polypeptide chains but can be cleaved into two active peptides (C5a and C5b) by C5 convertases. Mutations and defects in C5 are associated with severe recurrent infections.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C5, C5a anaphylatoxin, Complement C5, Complement C5 alpha chain, Complement C5 alpha' chain |
