验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, L02 cells, SH-SY5Y cells, NIH/3T3 cells, HSC-T6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87462-1-RR targets MUS81 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag1476 Product name: Recombinant human MUS81 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 202-551 aa of BC009999 Sequence: RYSLTPEGLELAQKLAESEGLSLLNVGIGPKEPPGEETAVPGAASAELASEAGVQQQPLELRPGEYRVLLCVDIGETRGGGHRPELLRELQRLHVTHTVRKLHVGDFVWVAQETNPRDPANPGELVLDHIVERKRLDDLCSSIIDGRFREQKFRLKRCGLERRVYLVEEHGSVHNLSLPESTLLQAVTNTQVIDGFFVKRTADIKESAAYLALLTRGLQRLYQGHTLRSRPWGTPGNPESGAMTSPNPLCSLLTFSDFNAGAIKNKAQSVREVFARQLMQVRGVSGEKAAALVDRYSTPASLLAAYDACATPKEQETLLSTIKCGRLQRNLGPALSRTLSQLYCSYGPLT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | MUS81 endonuclease homolog (S. cerevisiae) |
| 别名 | EC:3.1.22.- |
| 计算分子量 | 61 kDa |
| 观测分子量 | 52-72 kDa |
| GenBank蛋白编号 | BC009999 |
| 基因名称 | MUS81 |
| Gene ID (NCBI) | 80198 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q96NY9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Crossover junction endonuclease MUS81 has associated endonuclease activity against structure-specific oligonucleotide substrates, including synthetic Holliday junctions(PMID:11741546). It interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The open reading frame of human MUS81 predicts a translation product of 551 amino acids with a molecular mass of 59 kD. There are some reports showed that the enzyme can express a 72 kDa protein in human tissues(PMID:12724407).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MUS81 antibody 87462-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
