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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 25896-1-AP (same clone as 25896-1-PBS)

    WB analysis using 25896-1-AP (same clone as 25896-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25896-1-AP (MTR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25896-1-PBS in a different storage buffer formulation.

  • WB analysis of A375 using 25896-1-AP (same clone as 25896-1-PBS)

    WB analysis of A375 using 25896-1-AP (same clone as 25896-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 25896-1-AP (MTR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25896-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 25896-1-AP (same clone as 25896-1-PBS)

    IHC staining of human cervical cancer using 25896-1-AP (same clone as 25896-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25896-1-AP (MTR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25896-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTR antibody included
  • IHC staining of human cervical cancer using 25896-1-AP (same clone as 25896-1-PBS)

    IHC staining of human cervical cancer using 25896-1-AP (same clone as 25896-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25896-1-AP (MTR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25896-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTR antibody included
  • IF Staining of A375 using 25896-1-AP (same clone as 25896-1-PBS)

    IF Staining of A375 using 25896-1-AP (same clone as 25896-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using 25896-1-AP (MTR antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25896-1-PBS in a different storage buffer formulation.

MTR Polyclonal antibody, PBS Only

MTR Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25896-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Methionine synthase, 5-methyltetrahydrofolate--homocysteine methyltransferase, cblG, Cobalamin-dependent methionine synthase, EC:2.1.1.13

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MTR Ready-To-Use IHC Kit. MTR primary antibody included.

产品信息

25896-1-PBS targets MTR in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag23156

Product name: Recombinant human MTR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 917-1265 aa of BC130616

Sequence: RQGHYESLKERRYLPLSQARKSGFQMDWLSEPHPVKPTFIGTQVFEDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFNDKTVGGEARKVYDDAHNMLNTLISQKKLRARGVVGFWPAQSIQDDIHLYAEAAVPQAAEPIATFYGLRQQAEKDSASTEPYYCLSDFIAPLHSGIRDYLGLFAVACFGVEELSKAYEDDGDDYSSIMVKALGDRLAEAFAEELHERVRRELWAYCGSEQLDVADLRRLRYKGIRPAPGYPSQPDHTEKLTMWRLADIEQSTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQVEDYALRKNISVAEVEKWLGPILGYDTD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 5-methyltetrahydrofolate-homocysteine methyltransferase
别名 Methionine synthase, 5-methyltetrahydrofolate--homocysteine methyltransferase, cblG, Cobalamin-dependent methionine synthase, EC:2.1.1.13
观测分子量 140 kDa
GenBank蛋白编号BC130616
基因名称 MTR
Gene ID (NCBI) 4548
RRIDAB_2880287
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99707
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MTR (Methionine synthase), one of two B12-dependent mammalian enzymes, catalyzes a methyl transfer from methyltetrahydrofolate to homocysteine, generating methionine and tetrahydrofolate (PMID: 9587027, 9013615). Methionine synthase levels in mammalian cells are influenced by folate status, and the ability of cells to grow in the absence of methionine is partly rate-limited by synthase activity levels. MTR loss depletes cells and tumors of reduced folate species (PMID: 34799699)